fluorescence spectra of cardiac myosin and in vivo experiment: studies on daunorubicin-induced cardiotoxicity

نویسندگان

yang liu synergy innovation center of biological peptide antidiabetics of hubei province, engineering technology research center of biological peptide antidiabetics of hubei province, department of pharmaceutical engineering, school of life science, wuchang university of technology, wuhan, hubei province 430223, china

chi chen synergy innovation center of biological peptide antidiabetics of hubei province, engineering technology research center of biological peptide antidiabetics of hubei province, department of pharmaceutical engineering, school of life science, wuchang university of technology, wuhan, hubei province 430223, china

xiaoxiang duan synergy innovation center of biological peptide antidiabetics of hubei province, engineering technology research center of biological peptide antidiabetics of hubei province, department of pharmaceutical engineering, school of life science, wuchang university of technology, wuhan, hubei province 430223, china

wenting ma synergy innovation center of biological peptide antidiabetics of hubei province, engineering technology research center of biological peptide antidiabetics of hubei province, department of pharmaceutical engineering, school of life science, wuchang university of technology, wuhan, hubei province 430223, china

چکیده

objective(s):the objective of this study was to investigate the interaction of daunorubicin (dnr) and cardiac myosin (cm) and the changes in mice hearts to exhibit dnr-induced cardiotoxicity . materials and methods:the interaction between dnr and cm was expressed using fluorescence quenching at ph 4.0-9.0 and 15-37 °c. dnr-induced cardiotoxicity was studied using in vivo experiment.  forty groups mice were used control group in which mice were treated with dnr orally, and three dnr-treated groups in which mice were injected intraperitoneally with dnr at seven bolus doses of 2.0, 4.0, and 6.0 mg/kg body weight, respectively. heart indices and myocardial enzyme levels were obtained by histopathological and biochemical analysis. results:the fluorescence quenching mechanism of dnr-cm complex was observed to be a static procedure at 20 °c (ph 7.4), and weakly acidic environment (ph 4.0-6.0) or higher temperature(30-37 °c) promoted the interaction between dnr and cm, causing variations in conformation and normal physiological functions of cm. thermodynamic studies demonstrated that the binding of dnr to cm was a spontaneous process driven by entropy. it also indicated that hydrophobic interaction and hydrogen bonds may play essential roles in the combination of dnr with cm. in addition, 4.0-6.0 mg/kg dnr-treated mice exhibited obvious histopathological lesion, increase in myocardial enzyme level, and reductions in blood cell count. conclusion:our results are valuable for better understanding the particular mode of dnr-cm interaction, and are important to have a deeper insight into the dnr-induced cardiotoxicity.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fluorescence spectra of cardiac myosin and in vivo experiment: studies on daunorubicin-induced cardiotoxicity

Objective(s):The objective of this study was to investigate the interaction of daunorubicin (DNR) and cardiac myosin (CM) and the changes in mice hearts to exhibit DNR-induced cardiotoxicity . Materials and Methods:The interaction between DNR and CM was expressed using fluorescence quenching at pH 4.0-9.0 and 15-37 °C. DNR-induced cardiotoxicity was studied using in vivo experiment.  Forty grou...

متن کامل

Fluorescence spectra of cardiac myosin and in vivo experiment: studies on daunorubicin-induced cardiotoxicity

OBJECTIVES The objective of this study was to investigate the interaction of daunorubicin (DNR) and cardiac myosin (CM) and the changes in mice hearts to exhibit DNR-induced cardiotoxicity. MATERIALS AND METHODS The interaction between DNR and CM was expressed using fluorescence quenching at pH 4.0-9.0 and 15-37 °C. DNR-induced cardiotoxicity was studied using in vivo experiment. Forty groups...

متن کامل

EPR and fluorescence depolarization studies on bovine cardiac myosin.

To test for possible differences in local conformation and S1 flexibility, bovine cardiac and rabbit skeletal myosins were labeled with a fluorophore (1,5-IAEDANS) and a spin label having iodoacetamide reactivity. The marked activation of the Ca2+-ATPase (6- to 8-fold) and inhibition of the K+ (EDTA)-ATPase (80-90%) by both labels indicated specific labeling of the fast-reacting thiols (SH1) of...

متن کامل

Carvedilol Attenuates Inflammatory-Mediated Cardiotoxicity in Daunorubicin-Induced Rats

Cardiotoxicity, which results from intense cardiac oxidative stress and inflammation, is the main limiting factor of the anthracyclines. Carvedilol, a beta blocker that is used as a multifunctional neurohormonal antagonist, has been shown to act not only as an anti-oxidant, but also as an anti-inflammatory drug. This study was designed to evaluate whether carvedilol exerts a protective role aga...

متن کامل

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

Carvedilol-Afforded Protection against Daunorubicin-Induced Cardiomyopathic Rats In Vivo: Effects on Cardiac Fibrosis and Hypertrophy

Anthracyclines, most powerful anticancer agents, suffer from their cardiotoxic effects, which may be due to the induction of oxidative stress. Carvedilol, a third-generation, nonselective β-adrenoreceptor antagonist, possesses both reactive oxygen species (ROS) scavenging and ROS suppressive effects. It showed protective effects against daunorubicin- (DNR-) induced cardiac toxicity by reducing ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۱۲، صفحات ۱۱۹۹-۱۲۰۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023